Bioluminescence assay of adenosine triphosphate in drug susceptibility testing of Mycobacterium tuberculosis .
Vanaja Kumar; Selvakumar, N.; Venkatesan, P.; Chandrasekaran, V.; Paramasivan, C.N.; Prabhakar, R.
Indian Journal of Medical Research; 1998; 107; 75-77.
Twenty-three clinical isolates of M. tuberculosis and the reference strain, M. tuberculosis H 37 R v were tested for their susceptibility to trifluoperazine (TFP) by the standard broth dilution method and the bioluminescence assay. The results showed that in 15 of the 23 isolates, the minimal inhibitory concentration (MIC) was identical in both the methods and in the remaining 8 isolates the difference in the MIC values between the methods, was less than two fold and was not significant. The findings suggest that the measurement of adenosine triphosphate (ATP) by biotuminescence assay can be employed as an alternative method for the rapid screening of clinical isolates for their susceptibility to anti-mycobacterial agent.
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